Always load tubes symmetrically in pairs. Tubes facing each other must be of equal weight.
Insert the emergency release tool (or a small screwdriver) into the opening to manually trip the mechanical latch. 6. Service and Calibration Updates
If you can provide the or specific issue you are facing, I can offer more tailored troubleshooting steps. eppendorf 5402 manual updated
If you are still working with the legendary Eppendorf 5402 centrifuge, it’s time to check your documentation. We’ve been looking over the updated manual, and it’s a great reminder of why this workhorse remains a staple in the lab.
| | Original Instruction | Updated Instruction (Rev. 5+) | |-----------|--------------------------|------------------------------------| | Rotor inspection | Visual check monthly | Visual + dye penetrant test every 6 months | | Lid lock test | Not mentioned | Perform microswitch continuity test before each use | | Temperature calibration | User not advised | Use external Pt100 sensor quarterly; offset adjustable via rear panel | | Emergency lid release | Mechanical pull cord | Mechanical pull cord + magnetic override code (in addendum) | Always load tubes symmetrically in pairs
The is a legacy refrigerated microcentrifuge model known for its reliability in handling 1.5 mL to 2.0 mL tubes at variable speeds . While specific "updated" official manuals are rare for this discontinued model, the core operational features and specifications remain consistent for maintenance and lab use. Core Specifications Maximum Speed: 14,000 RPM (approximately 16,000 x g) .
remains a common fixture in labs as a reliable refurbished unit for DNA precipitation and cell lysate separation . Variable Speed Range: Achieves speeds up to 14,000 rpm . We’ve been looking over the updated manual, and
Older centrifuge models like the 5402 often rely on a motor with brushes, a consumable part that wears down over time. Checking the brushes is a key maintenance task. Here’s the general procedure (always consult your manual):
Digital controls allow for run times between 1 and 99 minutes, plus a momentary "Short" spin mode. Operational Guidelines
